The experiment was conducted in a petri dish and aimed to answer the question of whether the mRNA in the vaccine could be converted to DNA. 2021 Feb 24;12:640093. BNT162b2 is another name for the Pfizer-BioNTech COVID-19 vaccine that is marketed under the brand name Comirnaty. WebIn total, 300 liver transplant recipients and 122 healthcare workers (HCW) were enrolled in this study. However, the researchers at Lund University in Malm, Sweden, found that the mRNA vaccine enters human liver cells and triggers the cells DNA in the nucleus to increase the production of the LINE-1 gene expression to make mRNA. doi: 10.1128/Spectrum.01162-21. Disclaimer. Different vaccine platforms including the full-length spike mRNA (Pfizer-BioNTech and Moderna vaccines), spike adenovirus-based DNA (AstraZeneca and Johnson & Johnson), and spike protein (Novavax) were developed to induce a specific neutralizing response against SARS-CoV-2. MR: One should consider that cell lines differ from cells in living organisms, and therefore it is important that similar investigations are also studied in humans. part may be reproduced without the written permission. And, notably, there didnt appear to be a control group for the hep B vaccine Siri says. This also explains the choice of vaccine concentrations in our study, something we specifically address in the paper, which are 0.52% of the injection site concentration. Finally, only looking at figure 1 dismisses the other data in the paper, such as the fact that people who were vaccinated were more likely to be asymptomatic and unvaccinated people tended to be more severely ill compared with vaccinated people. Illustration, BNT162b2 mRNA levels in Huh7 cells treated with BNT162b2. But studies are showing that is not the case. Additionally, the mRNA vaccine does in fact travel to the liver and other locations such as ovaries. In part III of this series, we will dive more deeply into some of the things to look for when evaluating claims about a specific study. (AP Photo/Jae C. Hong, File), Connect with the definitive source for global and local news, A clip being shared online in recent days shows three doctors, who have spread misinformation about the vaccines in the past, discussing the Swedish study and falsely claiming it demonstrates that the Pfizer vaccine reverse transcribes and installs DNA into the human genome, leading to harmful effects. Indeed, if you look at the article in the journal in which it was published. In the August 2022 James Gill paper titled Autopsy Histopathologic Cardiac Findings in 2 adolescents following the second COVID-19 vaccine dose, two teenage May 03, 2022 These cells have had the normal protections of the immune system removed.. (In-vitro Swedish Study) An accepted and published Swedish study demonstrates for the Alden M, Falla FO, Yang D, et al. They are not sure if it gets integrated in our DNA or not. They used different concentrations of the vaccine and Detection of DNA amplicons of BNT162b2 in Huh7 cells treated with BNT162b2. N. Engl. -. J Transl Autoimmun. Immunohistochemistry using antibody binding to LINE-1 open reading frame-1 RNA-binding protein (ORFp1) on Huh7 cells treated with BNT162b2 indicated increased nucleus distribution of LINE-1. 2022 Apr;28(4):612.e1-612.e7. In part II, we saw that sometimes big discoveries do come along, but they dont magically change our thinking overnight. Consequently, other than the immunization, there are no permanent changes. Further, the newest vaccines are personalized, meaning they can target a patients Selena Gomez. The results have been widely discussed across social mediabut the results have in many cases been misinterpreted. Our publication is the first in vitro study on the conversion of mRNA vaccine into DNA, inside cells of human origin. The CDC says that the COVID-19 vaccines do not change or interact with your DNA in any way, claiming that all of the ingredients in both mRNA and viral vector COVID-19 vaccines (administered in the United States) are discarded from the body once antibodies are produced. The researchers then investigated how different gene expressions in the cells changed over time. badbunny. J. Med. Why liver cells and why the specific dose? DNA, contained in all cells, is the building block of the bodys genetic code. Because the study design does not necessarily reflect what happens in most human bodies, he said the findings cannot be extrapolated to make inferences about what would happen in human subjects. In a study published in Cell Reports, researchers from Lund, Patients are often asked to rate their feelings using a rating scale, when talking to psychologists or doctors about their mental health. Finally, as the authors pointed out, expression of LINE-1 has been shown to increase during viral infections, including with SARS-CoV-2 virus. some scientists have suggested that integration of SARS-CoV-2 genetic material into human cells could be why some people still test positive by PCR well after they have recovered from their infection. BNT: BNT162b2; L: DNA ladder; Ctrl1: cultured Huh7 cells; Ctrl2: Huh7 cells without BNT162b2 treatment collected at 6 h; Ctrl3: Huh7 cells without BNT162b2 treatment collected at 24 h; Ctrl4: Huh7 cells without BNT162b2 treatment collected at 48 h; Ctrl5: RNA from Huh7 cells treated with 0.5 g/mL of BNT162b2 for 6 h; Ctrl6: RNA from Huh7 cells treated with 0.5 g/mL of BNT162b2 for 6 h, digested with RNase. doi: 10.7759/cureus.32361. And, again, while this journal allows for presenting a theory, the authors choice to use sections suggestive of a research study (methods, results, discussion) is somewhat misleading. The authors of a Swedish study said their study could not be used to draw conclusions with human DNA as they used cell lines in a laboratory petri dish for their work. All quotes delayed a minimum of 15 minutes. Huh7 cells were treated without (Ctrl) or with 0.5 g/mL of BNT162b2 for 6, 24, and 48 h. Genomic DNA was purified and digested with 100 g/mL RNase. 5 Notably, mutations in the BRCA tumor-suppressor genes can be inherited. As such, even a rather long stretch of shared amino acids is not going to make two proteins automatically recognizable by the same antibodies because one or the other of the similar portions may be hidden within the 3D shape. Imaging findings included inflammation, beading, stricturing, and dilation of the biliary tree. YDM: More research is needed. No information in this video should ever be considered as a substitute for advice from a healthcare professional.\r\rURL list from Friday, Feb. 25 2022\r\rCIMB | Free Full-Text | Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro in Human Liver Cell Line | HTML\rhttps://www.mdpi.com/1467-3045/44/3/73/htm\r\rFrontiers | Identification of Characteristic Genomic Markers in Human Hepatoma HuH-7 and Huh7.5.1-8 Cell Lines | Genetics\rhttps://www.frontiersin.org/articles/10.3389/fgene.2020.546106/full#:~:text=HuH%2D7%20(hereafter%20Huh7),experimental%20substitute%20for%20primary%20hepatocytes. 5. 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Virological and serological kinetics of SARS-CoV-2 Delta variant vaccine breakthrough infections: a multicentre cohort study. During the study, the researchers were able to detect DNA that had been converted from the vaccines mRNA in the lab-modified cell. Pfizer didnt comment on the findings of the Swedish study and said only that its mRNA vaccine does not alter the human genome. And this could be seen with natural virus infection as well, Moore said. Neither your address nor the recipient's address will be used for any other purpose. In a lab environment, i.e. Virological and serological kinetics of SARS-CoV-2 Delta variant vaccine breakthrough infections: a multicentre cohort study. We detected high levels of BNT162b2 in Huh7 cells and changes in gene expression of long interspersed nuclear element-1 (LINE-1), which is an endogenous reverse transcriptase. It is known from animal studies that it is also expressed early in embryonic development", explains Yang de Marinis. Phase I/II study of COVID-19 RNA vaccine BNT162b1 in adults. The patient We have a new vaccine, and it needs to be tested in cell and animal models and also in humans, in various ways. Unable to load your collection due to an error, Unable to load your delegates due to an error. CLAIM: A Swedish study shows that Pfizers COVID-19 vaccine changes recipients DNA. followers 26 videos. Drugs and Lactation Database (LactMed) [Internet]. Daily science news on research developments and the latest scientific innovations, The latest engineering, electronics and technology advances, The most comprehensive sci-tech news coverage on the web. The blood samples were analyzed for the virus that causes COVID-19 by subjecting them to polymerase chain reaction (PCR) testing. Does the Pfizer-BioNTech mRNA vaccine get converted to DNA or not? Indeed, about 8 percent of human DNA comes from viruses inserted into our genomes during evolution. Please select the most appropriate category to facilitate processing of your request. RNA levels of BNT162b2 are presented as logged 2, Immunohistochemistry of Huh7 cells treated with BNT162b2 on LINE-1 protein distribution. Further proof with COVID-19. YDM: One of the limitations of our study is that we dont know if what we observed in this cell line could also happen in cells of other tissue types, and this needs to be addressed in follow-up studies. Published on The content provided in this video is for informational and educational purposes only.\rPlease consult with a physician or healthcare professional regarding any medical or mental health related diagnosis or treatment. The results have been widely discussed across social media but the results have in many cases been misinterpreted. We also show that BNT162b2 mRNA is reverse transcribed intracellularly into DNA in as fast as 6 h upon BNT162b2 exposure. doi: 10.1056/NEJMoa2034577. (In-vitro Swedish Study) https://youtube.com/live/MjxlvduyJyc?feature=share 28 Feb 2023 02:39:58 The dots are placed based on the day of illness (x-axis) and something called cycle threshold, which is a measure of the number of cycles in PCR that it took to detect viral RNA (y-axis). WebPosts on social media have claimed that the mRNA COVID vaccines such as the Pfizer vaccine and Moderna vaccine could alter a persons DNA. Keywords: Some genetic mutations are inherited in the DNA that parents pass on to their children at birth, including gene changes that are known to increase the risk of developing cancer. All versions of Internet Explorer are no longer supported, either by us or Microsoft (read more here: WebThe interaction between pre-existing liver disease and coronavirus infection has been illustrated, whereby coronaviruses influence the occurrence, severity, prognosis and treatment of liver diseases. Each dot on the table represents a study subject. The researchers write that the findings warrant a further As with the Ricke paper, this report presents a theory but not evidence of something happening. Sudden deaths of Canadian The antibody binds to the pathogen, but instead of blocking it from entering cells, it facilitates entry of the pathogen into cells. , ! YDM: A previous study from MIT has indicated that the SARS-CoV-2 virus mRNA can be converted to DNA and integrated into the human genome. On the figure, the two lines (comparing vaccinated and unvaccinated patient data) are almost in the same place for the first four or five days of illness, but then they separate, and the vaccinated group more quickly requires higher numbers of PCR cycles to detect viral RNA, meaning there was less virus in those samples. Genomics, diabetes and endocrinologyyang [dot] de_marinis [at] med [dot] lu [dot] se, Box 117, 221 00 Lund, Sweden 5. The text in one post reads: BOMBSHELL: Biological Study PROVES Pfizer mRNA Vaccine Permanently Alters Human DNA. In thisstudywe present evidence that COVID-19 mRNA vaccine BNT162b2 is able to enter the human liver cell line Huh7 in vitro, the researchers wrote in the study, published inCurrent Issues of Molecular Biology. 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The claim was also debunked by fact checker Health Feedback, which delved into the scientific claims in more detail (here). About this website WebThis leads to immune responses that ultimately destroy those cells. The Study Has Received A Lot of Media Attention, What Are Your Thoughts on That? (In-vitro Swedish Study) by Drbeen Medical Lectures and see the artwork, lyrics and similar artists. CLAIM: A Swedish study shows that Pfizer's COVID-19 vaccine changes recipients' DNA. Our results indicate a fast up-take of BNT162b2 into human liver cell line Huh7, leading to changes in LINE-1 expression and distribution. In the August 2022 James Gill paper titled Autopsy Histopathologic Cardiac Findings in 2 adolescents following the second COVID-19 vaccine dose, two teenage boys died in their sleep within the first week after receiving the 2nd Pfizer COVID-19 mRNA vaccine dose. By Meiling Lee. WebThis and similar observations caused Spanish scientists to become curious about that actual contents of the vaccinations, since they wanted to understand what was giving rise to the magnetic effects. Intracellular reverse transcription of Pfizer BioNTech COVID-19 mRNA vaccine BNT162b2 in vitro in human liver. The Japanese regulatory agencys biodistribution study (pdf) of the Pfizer vaccine showed that some of the mRNAs moved from the injection site and through the bloodstream, and were found in various organs such as the liver, spleen, adrenal glands, and ovaries of rats 48 hours following injection. We show that the vaccine enters liver cells as early as six hours after the vaccine has been administered. Professor at Infection Medicine Pfizer did not comment on the studys findings, the Epoch Times reported, stating only that its mRNA vaccine does not alter the human genome. This is 1 replication competent plasmid per 350 mRNA molecules and equates to billions of antibiotic resistant plasmids injected per person per shot . Anti-vaccination and ivermectin proponents have gone bananas over the new study from the Lund University This article was produced by the Reuters Fact Check team. followers 129 videos. Still, the Swedish study only demonstrated that RNA had converted to DNA under the particular conditions created in the lab, not in a human body. Pfizer Vaccine Becomes DNA in Liver Cells. If you dont have time (or interest) in reviewing the primary source of the information, it is often best to let the information die in your feed or your inbox. A chocolaty goodness, an amazing comforting breakfast dish that the whole family will love! The result might be surprising, but it is also a bit surprising that such studies do not seem to have been carried out before. Whether our results are true for other cell types in humans, or if they are specific to mRNA vaccines, are among many questions for further research. These findings were observed in petri dishes under experimental conditions, but we do not yet know if the converted DNA is integrated into the cells DNA in the genome - and if so, if it has any consequences, Rasmussen says in the article. As such, by definition, a modeling study cannot serve as proof. The authors used a cancerous liver cell line. Comment on Aldn et al. The authors also pointed out that this cell line has been shown to have genetic and protein expression differences specific to RNA metabolism (p. 1123). Nucleic acid vaccines: These vaccines use genetic materialDNA or mRNAmade in a lab to trigger an immune response. Two different antibody-dependent enhancement (ADE) risks for SARS-CoV-2 antibodies. The American Association of Liver Disease has recently published consensus statements for COVID-19 vaccination in subjects with chronic liver disease (CLD). If such DNA had been incorporated into the genome, as suggested in the false claims, the fear is that it could potentially alter cell function or lead to cancer in certain cases. PCR primer set used to detect mRNA level and reverse-transcription of BNT162b2. We saw that there was DNA converted from the vaccine's mRNA in the host cells we studied. The approval came as an emergency use authorization on December 11, 2020, after clinical trials showed the vaccine was 95% effective in preventing COVID-19. 2022 Nov;28(11):2273-2287. doi: 10.1038/s41591-022-02061-1. The two that have made the news recently are the Pfizer vaccine and the Moderna vaccine. This is part of APs effort to address widely shared misinformation, including work with outside companies and organizations to add factual context to misleading content that is circulating online. The information you enter will appear in your e-mail message and is not retained by Medical Xpress in any form. Well do this by highlighting a few papers that caused fear, generated confusion, or spread misinformation by looking at the interpretation, what it was based on, and why it was not correct. Illustration of BNT162b2 was adapted from previously described literature [34]. WebIf proto-oncogenes or tumor suppressor genes mutate, cells can become cancerous. Press Office Researchers found that the entire process of the Pfizer COVID vaccine becoming DNA in liver cells took about six hours. The research was conducted at Lund University. Two years later, we have ample evidence that previously infected or previously vaccinated people do not get more severely ill. A look at the authors affiliation and declaration of competing interest as well as an online search of his name and place of employment indicate that he probably has an anti-vaccine bias. In the general population, the antibody response wanes over the course of time after two doses of the vaccination, but seems to be more A new study from Lund University in Sweden on how the Pfizer-BioNTech COVID-19 vaccine affects human liver cells under experimental conditions, has been viewed more than 800,000 times in just over a week. DNA, which makes up our genetic code, is larger, double stranded and very long. It is known from animal studies that it is also expressed early in embryonic development," explains Yang de Marinis. Organisation number: 202100-3211, Webmail This document is subject to copyright. It was assumed that the vaccines spike protein would remain mostly at the injection site and last up to several weeks like other proteins produced in the body. Differences in mRNA-1273 (Moderna) and BNT162b2 (Pfizer-BioNTech) SARS-CoV-2 vaccine immunogenicity among patients undergoing dialysis. The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). World Health Organization Coronavirus (COVID-19) Dashboard. Data, especially data from vaccinated humans, will hopefully sort out the question marks. doi: 10.1038/s41586-020-2639-4. Apart from any fair dealing for the purpose of private study or research, no Its the first time that researchers have shown in vitro or inside a petri dish how an mRNA vaccine is converted into DNA on a human liver cell line, the Epoch Times reported. As such, even if DNA representing the viral RNA was integrated into the cell, no other cells with the altered DNA would be produced. Greece leads the car sales race in the EU! J. Med. These vaccines deliver genetic material that instructs cells to begin making spike proteins found on the surface of SARS-CoV-2 that causes COVID-19 to produce an immune response. The mRNA in the COVID-19 vaccines helps train the body to recognize a protein from the coronavirus to trigger an immune response. Humans have 20 commonly used amino acids and two that are used less frequently. The result might be surprising, but it is also a bit surprising that such studies do not seem to have been carried out before. DNA amplicons (444 bps) were visualized on agarose gel. By using our site, you acknowledge that you have read and understand our Privacy Policy doi: 10.1056/NEJMoa2027906. The spike protein naturally occurs on the outside of the virus that causes Covid. They have somewhere between 90 to 95% efficacy at providing immunity to someone Some genetic mutations are inherited in the DNA that parents pass on to their children at birth, including gene changes that are known to increase the risk of developing cancer. 2022 Dec 9;14(12):e32361. YDM: This study does not investigate whether the Pfizer vaccine alters our genome. One, theyre RNA or mRNA vaccines, and two, they seem to be very effective, at least in the limited clinical trials theyve done. DOI: 10.3390/cimb44030073. The site is secure. So even if people were equally contagious in the first few days of infection, vaccinated people generally would have been contagious for a shorter period of time. Please enable it to take advantage of the complete set of features! 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Spike proteins may circulate in the body after an infection or injection with a COVID-19 vaccine. Kids will surely love it Serve with crusty bread! Unauthorized use of these marks is strictly prohibited. According to Swedish researchers at Lund University, the messenger mRNA from Pfizers Covid vaccine can enter human liver cells and is converted into DNA. Study: Pfizer Covid Vaccine Converts to DNA Inside Liver Cells. MR: We understood that the study would attract attention, but we think it is self-evident that this type of research should be pursued. No information in this video should ever be considered as a substitute for advice from a healthcare professional.\r\rURL list from Friday, Feb. 25 2022\r\rCIMB | Free Full-Text | Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro in Human Liver Cell Line | HTML\rhttps://www.mdpi.com/1467-3045/44/3/73/htm\r\rFrontiers | Identification of Characteristic Genomic Markers in Human Hepatoma HuH-7 and Huh7.5.1-8 Cell Lines | Genetics\rhttps://www.frontiersin.org/articles/10.3389/fgene.2020.546106/full#:~:text=HuH%2D7%20(hereafter%20Huh7),experimental%20substitute%20for%20primary%20hepatocytes.\r\rURL list from Monday, Feb. 28 2022\r\rCIMB | Free Full-Text | Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro in Human Liver Cell Line | HTML\rhttps://www.mdpi.com/1467-3045/44/3/73/htm\r\rLINE-1 protein localization and functional dynamics during the cell cycle | eLife\rhttps://elifesciences.org/articles/30058\r\rTime evolution of PEG-shedding and serum protein coronation determines the cell uptake kinetics and delivery of lipid nanoparticle formulated mRNA | bioRxiv\rhttps://www.biorxiv.org/content/10.1101/2021.08.20.457104v1\r\rReverse-transcribed SARS-CoV-2 RNA can integrate into the genome of cultured human cells and can be expressed in patient-derived tissues - PubMed\rhttps://pubmed.ncbi.nlm.nih.gov/33958444/\r\rNo evidence of SARS-CoV-2 reverse transcription and integration as the origin of chimeric transcripts in patient tissues - PMC\rhttps://www.ncbi.nlm.nih.gov/labs/pmc/articles/PMC8379926/\r\rOverview of Reverse Transcription - Retroviruses - NCBI Bookshelf\rhttps://www.ncbi.nlm.nih.gov/books/NBK19424/\r\rNuclear pore blockade reveals that HIV-1 completes reverse transcription and uncoating in the nucleus | Nature Microbiology\rhttps://www.nature.com/articles/s41564-020-0735-8\r\rChimeric RNA - Wikipedia\rhttps://en.wikipedia.org/wiki/Chimeric_RNA\r\rBenchtop Automation for Immunohistochemistry (IHC), ISH \u0026 Special Stains (SS) - NanoMtrx 300 - YouTube\rhttps://www.youtube.com/watch?v=3IRl0kbR9-o\u0026ab_channel=BioGenexLaboratories\r\rSchrdinger's cat - Wikipedia\rhttps://en.wikipedia.org/wiki/Schr%C3%B6dinger%27s_cat 2020;586:589593. And, this month, we saw a few of the many ways that science can be misinterpreted and misused. Rasmussen also said, cell lines differ from cells in living organisms, and therefore it is important that similar investigations are also studied in humans..

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